This kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA). This kit is intended for use in investigative research only and not for human clinical diagnostic use.
This Histamine ELISA kit (Life Science Format) should not be used for determining histamine levels in scombroid fish. Neogen offers a separate kit for determination of histamine in fish and other foods. Intended User: Researchers in biomedical fields.
Neogen’s Histamine ELISA test kit (Life Science Format) is a competitive direct ELISA (Enzyme-Linked Immunosorbent Assay) in a microwell format that allows users to obtain exact concentrations of histamine in nanograms per milliliter.
The microwells in this assay kit are pre-coated with a monoclonal antibody to histamine. The sample or standard solution is first added to the antibody coated microplate. Next, the enzyme conjugate is added, and the mixture is shaken and incubated at room temperature for 45 minutes. During the incubation, unbound (free) histamine in the samples or standards compete with enzyme (horseradish peroxidase: HRP)-labeled histamine (conjugate) for antibody binding sites. The plate is then washed, removing all the unbound material. The bound enzyme conjugate is detected by the addition of a one-component peroxidase substrate which generates color by horseradish peroxidase. An optimal color is generated after 30 minutes. A microplate reader is then used to take an absorbance reading at 650 nm.
Quantitative test results may be obtained by measuring and comparing the absorbance reading of the sample wells against the standard curve using a log-logit curve fitting model. The extent of color development is inversely proportional to the amount of histamine in the sample or standard. For example, the absence of histamine in the sample will result in a bright blue color, whereas the presence of histamine will result in decreased or no color development.
Standard Curve in EIA Buffer
|Product #||Product Description|
|409010||Histamine Kit, 96 well kit|
Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.
Calculation of Results
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For research use only.