Neogen ELISA Drug Detection KitNeogen Corporation’s Methotrexate ELISA (Enzyme-Linked ImmunoSorbent Assay) test kit is a qualitative one-step kit designed for use as a screening device for the detection of Methotrexate. The kit was designed for screening purposes and is intended for forensic use only.

Intended Use

For the determination of trace quantities of Methotrexate and/or other metabolites in human urine, blood or oral fluid. Contact a Neogen representative for information on other matrices.


Compound I-50 in EIA Buffer
Methotrexate 0.22 ng/mL
Aminopterin 0.66 ng/mL
Triamterene 29.7 ng/mL

Specificity (Cross-Reactivity)

Compound Cross-Reactivity
Methotrexate 100%
Aminopterin 33.33%
Triamterene 0.74%
Folic Acid 0.002%
Trimethoprim 0.002%

Order Details

Product # Product Description
107519 Methotrexate, 96 well kit
107516 Methotrexate, 480 well kit


For forensic use only.

Please check with your local representative for availability of these products within your region.