Neogen ELISA Drug Detection KitLipoxin A4 (LXA4) is a biologically active lipoxygenase interaction product derived from arachidonic acid. Arachidonic acid is first oxygenated by 15-lipoxygenase to form 15-HETE which is then converted by 5-lipoxygenase and epoxide hydrase to generate LXA4. LXA4 stimulates leukocyte chemotaxis without aggregation and inhibits natural killer cell cytotoxicity. It also provokes contraction of parenchymal strips and stimulates microvascular changes. Recent findings indicate that LXA4 inhibits leukocyte-dependent inflammation. Determination of LXA4 levels may provide new understanding of the role of LXA4 in basic cellular reactions and in pathophysiology of inflammation and other disease processes.

This is an ELISA (Enzyme-Linked ImmunoSorbent Assay) for the quantitative analysis of Lipoxin A4 levels in biological fluid. This test kit operates based on competition between the enzyme conjugate and the Lipoxin A4 in the sample for a limited number of antibody binding sites.

The sample or standard solution is added to the microplate. Next, the diluted enzyme conjugate is added, and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650 nm. The extent of color development is inversely proportional to the amount of Lipoxin A4 in the sample or standard. For example, the absence of Lipoxin A4 in the sample will result in a bright blue color, whereas the presence of Lipoxin A4 will result in decreased or no color development.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Lipoxin A4 100%
15-epi-Lipoxin A4 24%
5(S), 6(R)-DiHete 5%
Lipoxin B4 1%
15-hete 0.10%
5-hete 0.10%
12-hete < 0.10%
Leukotriene B4 < 0.01%
Leukotriene C4 < 0.01%
Leukotriene D4 < 0.01%
Leukotriene E4 < 0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.04 ng/mL
   50% B/B0 = 0.15 ng/mL
Assay Range: 0.02 - 2.0 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions:  -20°C for lyophilized conjugate, 4°C for all other kit components
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
407010 Lipoxin A4 Kit, 96 well kit

Reference Materials

407010 - Lipoxin A4, Kit Insert [English]
407010 - Lipoxin A4, Safety Data Sheet [English]

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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