Neogen®’s Histamine ELISA test kit is designed for in vitro quantification of histamine in various biological fluids by competitive direct enzyme-linked immunosorbent assay (CD-ELISA). This kit is intended for use in research only.
The microwells in this assay kit are pre-coated with a monoclonal antibody to histamine. The sample or standard solution is first added to the antibody coated microplate. Next, the enzyme conjugate is added, and the mixture is shaken and incubated at room temperature for 45 minutes. During the incubation, unbound (free) histamine in the samples or standards compete with enzyme (horseradish peroxidase: HRP)-labeled histamine (conjugate) for antibody binding sites. The plate is then washed, removing all the unbound material. The bound enzyme conjugate is detected by the addition of a one-component peroxidase substrate which generates color by horseradish peroxidase. An optimal color is generated after 30 minutes. A microplate reader is then used to take an absorbance reading at 650 nm.
Quantitative test results may be obtained by measuring and comparing the absorbance reading of the sample wells against the standard curve using a log-logit curve fitting model. The extent of color development is inversely proportional to the amount of histamine in the sample or standard. For example, the absence of histamine in the sample will result in a bright blue color, whereas the presence of histamine will result in decreased or no color development.
Note: Neogen offers a separate kit for determination of histamine in fish and other foods.
Standard Curve in EIA Buffer
|Product #||Product Description|
|409010||Histamine Kit, 96 well kit|
Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.
Calculation of Results
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Follow the simple step-by-step guide to generate your data.
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For research use only.