Neogen ELISA Histamine KitCorticosterone is a glucocorticoid secreted by the adrenal cortex in response to stress. Glucocorticoids are also essential for proper metabolism of fats, proteins, and carbohydrates in the body. While cortisol and corticosterone are both produced in response to stress in humans, corticosterone is the predominant glucocorticoid produced in mice and rats. Corticosterone has also been used as a predictor of stress in a variety of wild animals. In particular corticosterone levels were increased in Galapagos marine iguanas under famine conditions brought on by El Niño.

While not always predictive of environmental stress in the wild, conditions such as starvation, rapid temperature change, or changes in daily routine can cause increased corticosterone levels in laboratory mice and rats.

Corticosterone is also administered to treat inflammation because like many other steroids, it is a powerful anti-inflammatory agent.

This is an ELISA (Enzyme-Linked ImmunoSorbent Assay) for the quantitative analysis of Corticosterone levels in biological fluid. This test kit operates based on competition between the enzyme conjugate and the Corticosterone in the sample for a limited number of binding sites on the antibody coated plate.

The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650 nm. The extent of color development is inversely proportional to the amount of Corticosterone in the sample or standard. For example, the absence of Corticosterone in the sample will result in a bright blue color, whereas the presence of Corticosterone will result in decreased or no color development.

Standard Curve in EIA Buffer

Curve in EIA Buffer

Specificity (Cross-Reactivity)

Analytes Cross-Reactivity
Corticosterone 100%
Deoxycorticosterone 38%
6-Hydroxycorticosterone 19%
Progesterone 5.1%
Tetrahydrocorticosterone 2.7%
Prednisolone 1.5%
Cortisol 1.1%
Pregnenolone 0.85%
11-Epicorticosterone 0.78%
Cortisone 0.27%
21-Desoxycortisol 0.27%
d-Aldosterone 0.13%
Testosterone 0.12%
17α-Hydroxyprogesterone 0.12%
Prednisone 0.10%
Dexamethasone 0.03%
Cholesterol < 0.01%
Estradiol < 0.01%
Estriol < 0.01%

Specifications

Sensitivity:
   80% B/B0 = 0.05 ng/mL
   50% B/B0 = 0.37 ng/mL
Assay Range: 0.05 - 5 ng/mL
Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 10%
Size: 96 well microplate
Assay Sample Size: 50 µL
Total Assay Incubation Time: 1.5 hours
Wavelength: 650 nm, 450 nm with acid stop
Storage Conditions: 4°C
Antibody: Polyclonal, Rabbit

Order Details

Product # Product Description
402810 Corticosterone Kit, 96 well kit

Reference Materials

402810 - Corticosterone, Kit Insert [English]
402810 - Corticosterone, Safety Data Sheet [English]

Kit inserts available on our website are for reference use only and are subject to change. It is always recommended to use the protocol included with the kit.

Calculation of Results

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